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Description

Materials and Methods Animals Five-week-old female C57BL/6 mice (2025 g) were utilized in this study

Blood 2010;116:1574-84

82000755) and Natural Science Foundation of Shandong Province (Grant No

Many patients drop inches and pounds without overhauling their lifestyle

For the observation of mitochondria generating ROS, the cells were incubated with HBSS (Sigma-Aldrich) containing 5 M MitoSOX for 10 min at 37 C in the dark and were then washed with PBS 3 times to remove any residual dye

For antigen retrieval, sections were heated in citrate buffer using microwave treatment for 3 minutes and then incubated in retrieval buffer for 10 minutes, followed by three PBS washes