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In a more in-depth study by Traunmller, L showed that correction of SLM2 exon defects rescued SLM2 knockout-induced synaptic plasticity and behavioral deficits [80]

Cells were lysed with 500 L of RIPA lysis buffer containing 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 25 mM Tris-HCl, supplemented with protease and phosphatase inhibitors at a 1:100 dilution