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DMEM/F12 (1:1) and FBS were gained from Gibco (Waltham, MA, USA)

et al., 2017)

Once confluent, the media was aspirated, cells were washed with 1X PBS, and incubated with 2 mL 0 or 10 mM NAC for 2 h

Fractions corresponding to the front, middle, and back parts of the Ub4 peak were collected separately

Findings from a cross-sectional analysis involving 450 adults diagnosed with MASLD revealed that greater adherence to the MIND diet was correlated with improved liver function, reduced systemic inflammation, enhanced metabolic health, as well as more favorable indicators of gut microbiome composition in MASLD patients

mTOR signaling factors downstream of IGF1R play key roles in the regulation of cancer cell metabolism, lipid and protein synthesis and they are responsible for several metabolic adaptations ( In vitro studies, over-expression of the IGF1R has been identified as a typical hallmark of many types of tumors (Werner et al., 2012)