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Afterwards, a gradual dehydration of cells was performed using ethanol at 30%, 50%, 70%, 95%, and 100%

PMID: 33843441 Nat Protoc2024 Jul;19(7):1984-2025

doi: 10.1186/2053-8871-1-10

Schedule: Initial series: 3 treatments spaced 46 weeks apart

Lysates were clarified by centrifugation at 15,000 g for 30 min at 4 C and flowed over pre-equilibrated Pierce high-capacity streptavidin agarose (3 ml of resin)

In certain embodiments, however, such recombinant human antibodies can be subjected to in vitro mutagenesis and thus the amino acid sequences of the V H and V L regions of the recombinant antibodies are sequences that, while derived from and related to human germline V- and V L sequences, may not naturally exist within the human antibody germline repertoire in vivo