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Reductase enzymatic activity The reductase activities of the WT and mutant (K8A, C20A and K47A) proteins were measured spectrophotometrically by recording the increase in absorbance at 260 nm using a UV-VIS spectrophotometer (UV-1800, Shimadzu, Kyoto, Japan) as described previously 37

4 UV radiation and visible light can cause lipid peroxidase in cellular membranes, causing the formation of free radicals that can stimulate melanogenesis

Those pharmacodynamic properties can be modulated by different modification and stabilization approaches (Vlieghe et al., 2010)

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