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In vulnerable people, this can become a tipping point

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Sperm suspensions held at 4C were then diluted slowly at 1:1 v/v (25L of sperm : 25L of cryoprotectant) in pre-cooled (4C) extender, either melatonin CPA, ascorbic acid CPA, uric acid CPA or control CPA over a period of 10 minutes with gentle homogenisation by flicking of the Eppendorf tube (Eppendorf Safe-Lock, Thermo Fisher Scientific)