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Once an entire biological set had been collected, worms were lysed through homogenization (four cycles of 30 seconds homogenization + 30 seconds in ice), followed by sonication (five cycles of 1 second at 10% amplitude with intervals of 30 seconds in ice)

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5.4 Prevention of DNA damage Oxidative stress is the major reason behind the majority of DNA damage in the case of human beings

In brief, MSCs were re-plated at a clonal density of 60 cells/well in six-well culture plates and cultured in hES-MSC medium for 14 days

10.1002/hep.28852

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