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APR encapsulated by niosomal gel showed a better strategy for high skin permeation and retention

The prevalence of use of individual medications and medication classes is shown in Figure 1

For LC-MS analysis, samples were thawed on ice-water bath, then 40 L of whole blood was injected into 450 L cold extraction solvent comprising of mixture of Acetonitrile:Methanol:Milli-Q water in ratio 40:40:20 according to protocol described in Presterud et al 35

Concentrations below 100mcg/mL require draw volumes exceeding 0.5mL for typical doses, necessitating larger 3mL syringes