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The positional orientation of the amide group relative to the cationic headgroup also influences transfection efficiency, with classical amide configurations, where the amine is proximal to the polar head, achieving efficiencies comparable to Lipofectamine 2000 across various cell types, whereas reverse isomeric amide bonds reduce efficiency due to increased molecular rigidity from Coulombic repulsion [114]

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3a, 400 M corticosterone significantly reduced the viability to 59.85 5.49% or 1.7-fold lower compared to negative control ( p 0.05)

This can be achieved either with a solution of a competing ligand or by changing the buffer conditions of the mobile phase, for example to high or low pH, elevated salt concentration, or by applying a chemical denaturant, detergent or chelating agent