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Pachymic acid, a competing activator of pyruvate kinase M2, mimics fructose-1,6-bisphosphate to blockage glycolysis and lactate production

Impact of LRRK2-specific ArfGAP1 phosphorylation sites on Golgi complex integrity ArfGAP1 is known to promote the GTP hydrolysis activity of Arf1 that is required for dissociation of the COPI coatmer complex from Golgi-derived vesicles, a prerequisite for vesicle fusion with target compartments such as the ER [3739]

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Here, we use a pooled, dual CRISPR screening strategy that knocks out pairs of transporters in four metabolic states glucose, galactose, OXPHOS inhibition, and absence of pyruvate designed to unmask the inter-dependence of these genes

Samples were completely lysed using an ultrasonic homogenizer in an ice bath for 20 min and centrifuged at 14,000 rpm under 4C for 10 min

You have simply created a lower concentration than intended, which means you will draw a larger volume per dose