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The herbs were dried at room temperature and stored at 4 C until use

Hydrogen sulfide and cell signaling

The detoxification against H 2 O 2 in mitochondria occurs mainly through the GSH redox system, including the glutathione peroxidases (Gpxs) and GSH reductases, as well as the presence of peroxiredoxins (Prxs

Homogenised lysates were incubated at 4 C under constant rotation for 2 h

Risk factors for riboflavin deficiency Alcoholics are at an increased risk of riboflavin deficiency, likely due to decreased dietary intake, decreased absorption, and/or impaired utilization of riboflavin

Reaction mixtures were incubated at 37 C for a desired amount of time (for H 2 S detection in the Jerome J605, 1 h with supraphysiological reaction mixture and 6 h with physiological reaction mixture