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Cells were cultured in suspension at 37 C with 5% CO and were passaged every 23 days to be in exponential growth, keeping cell density at 0.21 10 cells/ml

doi: 10.1016/j.diabres 4 CaoYLinJHHammesHPZhangC

By preventing this, glutathione helps protect against signs of ageing and maintain radiance

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Recent studies emphasize that overexpression alone does not fully explain metabolic resistance as factors including allelic variation, gene duplication, and interactions with co-factors like cytochrome P450 reductase (CPR) and cytochrome b5 (CYB5) also influence enzymatic activity and resistance intensity [194]

They work by reducing melanin production in the skin