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The LC flow was set at 0.2 ml min 1 , and the gradient was as follows: 10% A held for 2 min, 35% A at 18 min, 70% A at 22 min, 90% A at 22.1 min and held for 2.9 min, 10 % A at 25.1 min and held for 5 min

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Moreover, the structure of EcGGT in complex with glutathione clearly illustrates the formation of a covalent bond between the OG atom of Thr391 and carbonyl carbon of -glutamyl moiety of glutathione (Okada et al., 2006)

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