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Least significant difference (LSD) post hoc tests were used

These challenges include the short half-life and instability of the protein in vivo [106], the requirement of administering high volumes of endostatin to exert their anti-angiogenic effects, which in itself is associated with significant practical and cost implications [107], as well as technological challenges related to manufacturing a correctly folded and soluble protein to ensure adequate bioactivity within the tumour cells [108]

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A year later Paul Ehrlich, a German physician and scientist with expertise in structural chemistry, observed the same phenomenon and coined the term 'antibody' (German: Antikrper) for the newly found substance in blood

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