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The second fully expanded leaves from the shoot apex were harvested at the same time of day to reduce circadian effects, immediately frozen in liquid nitrogen, and stored at 80 C until analysis

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Also, -carotene supplementation was found to increase the risk of lung cancer in smokers regardless of the nicotine and tar content of cigarettes, indicating that smokers should refrain from taking this vitamin as a supplement unless they have vitamin A deficiency

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In ELISAs the antibody of interest does not have to be conjugated to a detectable compound