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Antioxidant activity and neuroprotective role of docosahexaenoic acid (Dha) supplementation in eye diseases that can Lead to blindness: a narrative review

Immunodetection was applied with primary antibodies specific to erythroid 2-related factor 2 (Nrf2) (1:5,000, Affinity, AF0639), phospho-Nrf2 (p-Nrf2) (1:1,000, Affinity, DF7519), superoxide dismutase 1 (SOD1) (1:1,000, Thermo, PA130195), glutathione peroxidase (GPx) (1:1,000, Abcam, Ab22604), catalase (1:2,000, Santa Cruz, sc-271803), NF-B (p-65) (1:250, Santa Cruz, sc-8008), TNF- (1:200, Santa Cruz, sc-52746), IL-6 (1:500, Bioss, bs-0782R-TR), IL-1 (1:100, Santa Cruz, sc-52012), COX2 (1:200, Santa Cruz, sc-376861), activating transcription factor 6 (ATF6) (1:200, Santa Cruz, sc-166659), inositol-requiring enzyme 1 (IRE1) (1:250, Santa Cruz, sc-390960), protein kinase R-like endoplasmic reticulum kinase (PERK) (1:100, Santa Cruz, sc-377400), caspase 3 (1:200, Santa Cruz, sc-56053), and -actin (1:5,000, Novus, NB600-501), respectively

T.et al (1999)

Once reconstituted with bacteriostatic water, the liquid solution must be kept refrigerated at all times

Functional Markers: MMA and Homocysteine Serum B12 is not the only way to assess vitamin B12 status

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