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ELISA for total IgG quantification Total serum IgG concentrations were measured using a commercial mouse IgG ELISA kit (PI480, Beyotime, Shanghai, China) according to the manufacturers protocol with minor adaptations

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Antithrombin and heparin bind to these factors and disable coagulation

Dermatol 21 (6), 813819

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Furthermore, investigating the relative degree of tumor dependency on exogenous glutamine at distinct stages of tumorigenesisthat is, in the primary tumor initiation and expansion, local tissue invasion, survival in the circulation and seeding, and colonization of distant organsmay provide further insights into the therapeutic potential of targeting glutamine metabolism in cancer therapy