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Description

Membranes were incubated overnight at 4 C with primary antibodies with anti-GCLC (1:1000, 12601-1-AP, Proteintech, China), anti-Nrf2(1:1000, ab62352, Abcam, UK), anti-NF-B (1:1000, ab32536, Abcam, UK), and anti--actin (1:1000, 66009-1-IG, Proteintech, China)

doi: 10.1371/journal.pone.0074957 21 MacphersonAJMcCoyKD

Preparation of the molecular setups, numerical analyses and visualizations were performed using Gromacs tools, PyMOL, and Visual Molecular Dynamics, as well as in-house Python and Bash scripts