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Abdel-Hadi AM, Awad MF, Abo-Dahab NF, Elkady MF

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The reaction mixture contained 0.005% BSA (Sigma), 1 mM reduced glutathione (Sigma), 100 M pyridoxal phosphate (Sigma), 100 M NADH (Sigma), 3 U/mL lactate dehydrogenase (Sigma) and apotryptophanase (10 U/mL, Sigma) in 0.15 M potassium phosphate buffer (pH 8.0)