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Total RNA was isolated from the cells using the easy-BLUE Total RNA Extraction kit (Intron Biotechnology Inc., Seongnam, Korea) and template RNA was amplified using 1 l RT reaction buffer, along with primers (Bioneer Corporation, Daejeon, Korea), dNTPs, and 0.5 U Taq DNA polymerase (Invitrogen Life Technologies, Carlsbad, CA, USA), in a final volume of 25 l

Science 299 , 117120 (2003)

Mood changes, including irritability, anxiety, or depressive symptoms, affect a small subset of users

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Start with the basics: eat your veggies, prioritize sleep, move your body, and manage stress

Preclinical studies demonstrate biologic activity relevant to tissue repair, supporting biologic plausibility but not clinical effectiveness