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They also found that 4-HNE production in OA articular tissues contributed to inflammatory responses by upregulating COX-2 expression and limiting the magnitude of transcriptional expression of iNOS [80]

For lentiviral production, Lenti-X 293T cells were seeded in six-well plates or 10-cm dishes and transfected at approximately 80% confluency with 0.6/3.4 g of target plasmid, 1.7/0.3 g of psPAX2 (Addgene, 12260) and 0.85/0.15 g of pMD2.G (Addgene, 12259) using PEI (PolySciences)