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Curcumin increases intracellular ROS levels and a treatment with an antioxidant partially reverses the anti-proliferative effects of curcumin Since many curcumin-binding candidates identified in this study (CBR1/3, GSTP1/O1, NQO1/2, AKR1C1/D1, PRDX1, GLO1, and ADH1A shown in Table 1) belong to the enzymes involved in the metabolism of ROS and oxidative-stress products (such as Reactive Carbonyl Species, RCS) 18 , and because curcumin inhibits the enzymatic activity of CBR1 19 , GSTP1 20 , NQO1 21 , and GLO1 22 in vitro , we speculated that the treatment with curcumin up-regulates intracellular ROS levels in CML-derived leukemic cells

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Antioxidant defense system During oxidative stress and other stress conditions increased levels of H 2 O 2 is associated with elevated production of antioxidant enzymes 31

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