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Subsequently, a small-animal ultrasound system (Vevo F2, Canada) equipped with a linear 46-MHz probe was utilized to obtain long-axis M-mode aortic images and to assess aortic blood flow velocity using Doppler mode

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[10] Long-term use of proton pump inhibitors (PPIs) and H2-receptor antagonists reduces gastric acid, potentially impairing the release of B12 from dietary proteins

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The duration depends on individual goals and response, typically ranging from several months to ongoing use