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doi: 10.1097/MNH.0000000000000160

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The FlowJo software was used to measure eGFP expression according to the following equation: MFI siGFP indicates the mean fluorescence intensity of cells treated with GFP-siRNA and MFI siCTRL indicates the mean fluorescence intensity of cells treated with negative control-siRNA

For metabolite extraction, 5 l of urine was transferred to a glass insert and extracted using 195 l of cold 80% methanol spiked with 500 nM metabolomics amino acid standard mix (Cambridge Isotopes Laboratory)

Murphy, 2020)

Following the transfer of aminopropyl groups, dcSAM transforms into 5-deoxy-5-methylthioadenosine, which then can undergo a series of steps in the Met salvage pathway to regenerate adenine and Met (Lauinger and Kaiser, 2021)