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S.VaresiA.FlorisV.SavioliG.MantovaniE.Lpez-CarrascoP.et al (2021)

L- Natural Health 598,2

I didnt see if it was one or more cells, but from experience going down to 3.1 or below makes it almost impossible to balance multiple cells ever again

Because these histidines are expected to be required for 9 desaturase activity we expressed this allele in mutant yeast that lack 9 desaturase activity ( ole1 mutants)

doi: 10.1073/pnas.2212476120 125 Azarcoya-BarreraJWollinBVeida-SilvaHMakarowskiAGorukSFieldCJet al

Therefore, the enzymatic activity of MSA-DH was initially shown in a coupled assay containing carnitine monooxygenase (5 M CntA and 15 M CntB) and 5 M MSA-DH in the presence of the respective cofactors NADH (10 mM) and NAD + (10 mM)