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Microscopy Microscopically, at 15 min after the beginning of ligation in IC rats, there was failed vascular presentation, with gross pale flat areas and the disappearance of mucosal folds (Figure 2)

Ellis FR, Nasser S

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The cell lysate was centrifuged (15,000 g for 30 min) at 4 C to remove the cell debris, and the resulting supernatant was loaded onto a Fast Flow Ni 2+ -agarose column (GE Healthcare, USA), which was pre-equilibrated with buffer A