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Description

Membrane was blocked overnight in 5% BSA in TBST on a shaker at 4 C

The animals were fed a normal diet and given water ad libitum

2014 Jan 1;55(1):46-58

doi: 10.1177/02601060251356147

The scaffold was soaked in an IGF-1 plasmid solution with a Lipofection reagent

Second, the search was expanded across three major academic databases: PubMed, Google Scholar, and Web of Science, ensuring broad coverage of both clinical and preclinical research