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Note that purifying His-tagged proteins from mammalian total cell lysates generally gives higher backgrounds than bacteria lysates because mammalian cells have a higher level of endogenous proteins with polyhistidine motifs

10.1038/nature25158 Nature 89 VirgD.Dalmadi-KissB.VkeyK.DrahosL.KlebovichI.AntalI.et al (2020)

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Here, we showed that RNK06138 treatment results in a decrease of ERK5 in both the cytosol and nucleus, with no significant activation of the MEF2 reporter (Fig

[DOI] [PMC free article] [PubMed] [Google Scholar] 69.Tury S., Assayag F., Bonin F., Chateau-Joubert S., Servely J.-L., Vacher S., Becette V., Caly M., Rapinat A., Gentien D., et al

After the break, continuing patients return to the month-three maintenance dose