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Trypsin and penicillin/streptomycin solution were obtained from PAN-Biotech GmbH (Germany)

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For DDM-solubilised samples, the supernatant was combined with sample buffer containing Coomassie G250, resulting in a final concentration of 0.25% DDM and 0.0625% Coomassie G250

Nevertheless, future studies using different doses of ALA, or continuous ALA administration throughout the post-CSD period are warranted to further enhance the translational value of the beneficial effects of ALA on counteracting the CSD-induced metabolic deficiency