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Cell cultures were subsequently incubated for 90 min at 37 C in 2 ml serum-free culture medium containing 2.5 mM [U- 13 C] glucose (neurons) or in the presence of 250 M [U- 13 C] glutamate plus 2.5 mM unlabeled glucose (neurons and astrocytes) or 0.5 mM [U- 13 C] glutamine (neurons) plus unlabeled glucose

Endocr Rev 33, 155-86 (2012)

It has been found that GPR41 mainly transmits signals by activating G protein of Gi/o family

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