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Unlike drug-induced ROS responses, KEAP1 loss causes persistent NRF2 signaling and increased glutathione synthesis via GCLC, GCLM and GSS 17

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459 , this review advances the field by providing an updated and mechanistically enriched synthesis

Total protein extracts were obtained by homogenization of tissues using protein sample buffer [100 mM Tris-HCl (pH 6.8), 200 mM dithiothreitol, 4% sodium dodecyl sulfate (SDS), 0.2% bromphenol blue and 20% glycerol] and a classic protease inhibitor cocktail (Beyotime Institute of Biotechnology)

Gomes, A., & Gomes, P

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