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Blando, Gerardi, and Nicoletti 2004)

Yes, in some cases they can be part of the same overall treatment plan, though not necessarily on the same area at the same time

10.1093/molbev/msw054 27 LegrandP.DumasR.SeuxM.RippertP.RavelliR.FerrerJ

doi: 10.1186/s12969-023-00908-6

For sequential double labeling, the explants were incubated with the second primary antibody MYO7A overnight at 4 C, followed by incubation with the second secondary antibody for 1 h at room temperature