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Primary hepatocytes were isolated from 810-week-old male C57BL/6J mice fed a standard chow diet as described below

Good technique matters

Similarly, chronic treatment with memantine, an uncompetitive/fast off-rate NMDA receptor antagonist, administered alone or in combination with the atypical antipsychotic drug aripiprazole, increased sociability and social novelty preference index in the three-chamber test, while alleviating repetitive behaviors and cognitive deficits in rats prenatally exposed to VPA [51]

Friedman SL

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Antidepressant medications are commonly used to treat anxiety, but acute anxiety may be treated with benzodiazepines