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Materials and methods Cell culture Cell lines were acquired from the American Type Culture Collection or the German Collection of Microorganisms and Cell Cultures and tested regularly for mycoplasma contamination

4.2 NOs role in mitochondrial dysfunction and oxidative stress The activity of mitochondrial nitric oxide synthase (mtNOS) is a key driver of mitochondrial dysfunction, oxidative stress, and progression in colorectal cancer (CRC)

The reaction rate was controlled for the effect of solvents DMSO (0.085%) and ethanol (120%) and no significant effect was observed under the experimental conditions

[2] The medications used for both groups were IV injections of dexamethasone over a five day period plus vitamin B1 and B12 intramuscular injections