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There are several anionic molecules in the membrane of cancer cells, such as O-glycosylated mucins, heparin sulfate, phosphatidylserine, and sialylated gangliosides, which give them a net negative charge while normal mammalian cell membrane is zwitterionic (29) Increasing the content of sialic acid on the membrane leads to enhancing the surface concentration of acidic groups and thus changing the membrane charge (140)