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[DOI] [PubMed] [Google Scholar] 84.Coombes JS, Rowell B, Dodd SL, Demirel HA, Naito H, Shanely RA, Powers SK

V., Grassucci, R

Otherwise low toxicity water-soluble

It was way back 5 years ago when i first tried this and took it for about 5 months

Cells were washed with ice-cold PBS, lysed in PBS containing 1% dodecyltrimethylammonium bromide in 0.2 M NaOH, and divided into two aliquotsone untreated and one treated with 1 M ascorbic acid followed by 0.4 M HCl