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In reaction mixture without enzyme solution, varying amounts of glutathione (0-300 l of stock solution giving 0-260 M final glutathione concentrations) were reacted with 150 l of monobromobimane solution (final concentration 50 M) in presence of phosphate buffer (3.0 ml) in cuvettes

Results were presented as mean SME

Parma, M., Stella Vanni, V., Bertini, M

doi: 10.3389/fenvs.2015.00025 Received 18 January 2015 Accepted 13 March 2015 Published 31 March 2015 Volume 3 - 2015 Edited by Naser A

Plasma glutathione levels peak 6090 minutes after oral administration of bioavailable forms and remain elevated for 46 hours before returning to baseline