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17 Under hypoxic conditions, a decrease in hydroxylation results in a reduction in the synthesis of the VHL and HIF-1 complex, which in turn promotes the stable production of HIF-1

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Apo- and mesohemerabbit HPX (90%) or proto-hemehuman HPX complexes (33.9 mM and 38.3 mM, respectively) were investigated

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Plates were washed five times with HBS + 0.1% Tween-20, and 100 l of streptavidinhorseradish peroxidase (HRP) was added to each well, followed by another one-hour incubation at room temperature