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doi:10.1039/c8bm01120f

Vander Heiden MG, DeBerardinis RJ

For metabolic analysis of cultured cancer cells and tumours, frozen tumour tissue was homogenized in 80% methanol containing 20 mM NEM using a Precellys cold tissue homogenizer (Bertin) at a ratio of 20 mg tissue to 1 ml solvent

Moreover, MZB entry and retention in the splenic marginal zone are tightly regulated by chemotactic molecules, such as C-X-C Motif Chemokine Ligand 13 (CXCL13) and sphingsoine-1-phosphate (S1P) 54

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To test the effect of Sp in paraquat-induced oxidative stress model, seven days old wild type flies, constitutively expressing mitochondrial (mt)-roGFP, were exposed to 10 mM paraquat for 24 h and then co-treated with 2.5 mM Sp for 24 h, prior to roGFP fluorescence estimation