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As detailed in a study by Kingma et al, early recognition through genetic testing and prompt initiation of parenteral vitamin B12 supplementation are crucial to prevent irreversible neurological damage and correct hematologic abnormalities

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Folic acid: Ingesting amounts greater than the Tolerable Upper Intake Level may mask vitamin B12 deficiency symptoms (33)

EdU click reaction was performed in buffer containing 100 mM Tris-HCl (pH 8.5), 2 mM CuSO 4 , 100 mM sodium ascorbate, and 5 M Alexa Fluor 488 Azide (Thermo Fisher Scientific, A10266) for 30 min in the dark followed by two washes with 1% (w/v) BSA in PBS