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Moreover, folic acid must be metabolized by dihydrofolate reductase (DHFR) to enter the folate cycle, with this reaction being slow and easily reaching saturation, thus limiting the therapeutic use of high doses of folic acid (Menezo et al ., 2022

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doi: 10.1016/j.freeradbiomed.2013.09.015

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Cells were washed twice with PBS and treated with 30 g ribonuclease A and 15 g of propidium iodide

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However, considering that the hiPSC-derived neurons are cultured in the presence of a higher concentration of glutamine (2 mM) than that used during the incubation experiments (0.5 mM), it is plausible that increased glutamine metabolism in FTD3 neurons is a compensatory mechanism to fuel mitochondria and potentially accelerate ROS production

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