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For OTUD5 modification with K29-linked ubiquitin using site-directed ubiquitylation 40 , full-length SUE1-FLAG-HA-OTUD5 (SUE1 sequence inserted into the N-terminus) was purified from 293 T cells using anti-FLAG antibody and eluted with 200 ng/mL FLAG peptide

365 In the resting state, T lymphocytes rely on catabolic pathways and autophagy to generate essential biomolecules for maintaining protein synthesis and energy production