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Figure 3 Effects of GTE on MMP-9 expression in lung tissue of OVA-induced asthmatic mice To evaluate MMP9 protein expression levels, IHC staining and western blotting of lung tissues were performed ( Figures 4A, B )

A least 2 weeks prior to tissue collection, a cohort of animals were maintained in a reverse light-dark cycle in which lights were turned off at 6am and lights were turned on at 6pm