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Protein concentrations were determined via BCA assay (Elabscience), with equal amounts loaded onto 4%20% gradient SDS-PAGE gels

The amounts of nuclear DNA (ncDNA) and mtDNA were quantified by RT-qPCR for both the beta-2-microglobulin (B2M) gene of ncDNA and the cytochrome-c-oxidase 1 (COX1) gene of mtDNA

doi: 10.1016/j.neuropharm.2008.08.010 53 HeSZhangYWangDTaoKZhangSWeiLet al

Semaphorins are large family of secreted proteins that helps in the cell dissociation and repulsive guidance events in situations like cell scattering during neural development