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Prior to analysis, the XFe96 instrument (Seahorse Biosciences, North Billerica, MA, USA) mixed the assay media in each well for 10 minutes to allow the oxygen partial pressure to reach equilibrium

The direct comparison upon using the same concentration of ruthenium of the molecular agent Ru(II)-OH and NP4 showed a significantly higher tumor accumulation of the nanoparticle formulation NP4 (Supplementary Fig