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The mammalian target of rapamycin (mTOR), a key protein downstream of the phosphatidylinositol 3-kinase/protein kinase (PI3K/AKT) pathway, is closely associated with insulin metabolic dysfunction and exerts a substantial impact on the growth and proliferation of FGCs

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This effect is achieved by promoting GDH1 expression through the downregulation of the epidermal growth factor receptor (EGFR)/MEK/ERK signaling pathway [56]

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GSNO was used solely as a nitrosylating agent to generate S-nitrosylated TTR, as previously shown [64], and excess reagent was removed prior to analysis