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Subsequently, the OCR was measured following the addition of 1 M oligomycin to inhibit ATP synthesis from oxidative phosphorylation, 1.5 M carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone (FCCP) to uncouple the mitochondrial membrane that stimulates respiration, and a mixture containing 0.5 M each of antimycin A and rotenone (A+R) to inhibit complex I and III that terminates mitochondrial oxidative phosphorylation

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