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Wyllie AH, Kerr JFR, Currie AR: Cell death: The significance of apoptosis

Evidence for a role of endothelin 1 and protein kinase C in nitroglycerin tolerance

Cells were lysed at 4 C for 2 h by adding 100 L of lysis buffer per well, which consisted of 90% methanol and 10% distilled water (v/v), containing 1 M taurine- 13 C 2 as an internal standard