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Under in vitro conditions, disruption of Syns tertiary ensemblesthrough charge neutralization (acidic pH or Ca 2+ addition), C-terminal truncation, or Syn-S129p could expose the hydrophobic (NAC) region and transform it into a metastable, reactive state 15,68

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To determine whether induced expression of IRF5 and IKK effectively reprogrammed macrophages from M2 to M1, researchers generated M2 macrophages by culturing bone marrow-derived macrophages (BMDMs) with IL-4