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Morphological changes, occurring during the activation of microglia and astrocytes, or higher proliferation of NG2 glia, microglia and astrocytes are all hallmarks of ischemia (Davalos et al., 2005

The samples were loaded on the 96-well S-Trap plate (Protifi), placed on top of a deepwell plate, and centrifuged for 2 min at 1,500 x g at RT

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